Since the 16S profiles from the gut look very unique for intracellular and extracellular DNA [33], qPCR is expected to mirror the NGS-focused Local community composition each quantitatively and qualitatively extra carefully than flow cytometry.
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The qPCR primers have a little reduce but adequately comparable coverage for your area Germs in comparison to the primers for NGS. The coverage of each qPCR and NGS primer sets is extremely equivalent for your four taxa chosen for taxon-unique qPCR (S1B Table), which offers reputable validation of the explained technique During this examine. For long run advancement with the herein offered technique, a qPCR assay utilizing the exact same primer pair as for NGS may be optimized and validated.
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